Replication Needed to Distinguish Alterations in Cell Ratios, the Frequency of Individual Stages of the Cycle of the Seminiferous Epithelium, or the Appearance of Abnormalities in the Testes of Rodents, Rabbits, or Humans

Abstract

The typical levels of inherent variability among untreated sexually mature rodents rabbits or men were determined for several endpoints used to characterize spermatogenesis The latter included germ cell germ cell and germ cell Sertoli cell ratios the frequency of specific stages of the cycle of the seminiferous epithelium and the appearance of a variety of testicular abnormal dies Based on this variability the number of replicates needed to provide future experiments of a predictable power and sensitivity was estimated Replication requirements differed greatly as a function of species and/or among specific endpoints within a single species In addition the replication needed to provide robust experiments was substantially greater than that employed in most investigations The application of these findings during the design and interpretation of future experiments was discussed

Publication Date

11-1-2010

Journal Title

Journal of andrology

Publisher

American Society of Andrology

Digital Object Identifier (DOI)

10.2164/jandrol.109.008920

Scientific Contribution Number

2393

Document Type

Article

Rights

Copyright © American Society of Andrology

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