Alloantigen system L affects the outcome of Rous sarcomas

Abstract

This study was designed to examine the alloantigen system L effects on Rous sarcomas in three B complex genotypes. The parental stock was 50% Modified Wisconsin Line 3 x White Leghorn Line NIU 4 and 50% inbred Line 6.15-5. Pedigree matings of two (BB5)-B-2 (LL2)-L-1 sires to five (BB5)-B-2 (LL2)-L-1 dams per sire produced experimental chicks segregating for B and L genotypes. Chicks were inoculated with 20 pock-forming units (pfu) of Rous sarcoma virus (RSV) at 6 weeks of age. Tumors were scored six times over 10 weeks postinoculation after which the tumor scores were used to assign a tumor profile index (TPI) to each chicken. Tumor growth over time and TPI were evaluated by repeated-measures analysis of variance and analysis of variance, respectively. Six trials were conducted with a total of 151 chickens. The major histocompatibility (B) complex affected the responses as the (BB2)-B-2 and (BB5)-B-2 genotypes had significantly lower tumor growth over time and TPI than the (BB5)-B-5 genotype. Separate analyses revealed no significant L system effect in (BB2)-B-2 or (BB5)-B-2 backgrounds. However, L genotype significantly affected (P < 0.05) both tumor growth over time and TPI in (BB5)-B-5 chickens. (BB5)-B-5 (LL2)-L-1 birds had TPI significantly lower than (BB5)-B-5 (LL1)-L-1 chickens but not (BB5)-B-5 (LL2)-L-2. Mortality was lower in the (BB5)-B-5 (LL2)-L-1 birds than in (BB5)-B-5 (LL2)-L-2 chickens. The L system, or one closely linked, affects the growth and ultimate outcome of Rous sarcomas. The response may depend upon the genetic background as well as MHC type.

Publication Date

3-1-2002

Journal Title

Experimental biology and medicine

Publisher

Sage Publications

Scientific Contribution Number

2080

Document Type

Article

Rights

Copyright © 2002 by the Society for Experimental Biology and Medicine

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