Differential Antibody Responses in 6.B Major Histocompatibility (B) Complex Congenic Chickens
Abstract
Lines 6.6-2 (B 2 B 2) and 6.15-5 (B 2 B 2), congenic for the major histocompatibility (B) complex with > 99.9% background gene uniformity, were used to examine primary antibody responses to two antigens. In each of two trials, 1 mL of 5% SRBC, a T cell-dependent antigen, or 0.1 mL of Brucella abortus (BA), a T cell-independent antigen, was injected into separate groups of each B genotype aged 3 and 6 wk. Blood samples were taken from the chickens 7 d after immunization. Serum titers (log2) for both total antibody and mercaptoethanol (ME)-sensitive antibody to detect IgG were assayed by microtiter procedures. Least squares analysis of variance and Fisher’s protected Least Significant Difference at P < 0.05 were used to evaluate the data. The total anti-SRBC antibody titer was significantly higher in B 5 B 5 chicks than in B 2 B 2 chicks at 4 and 7 w k of age. There was no significant difference in ME-sensitive antibody to SRBC. Chicks of the B 5 B 5 genotype also had significantly higher total and IgG antibody titers to BA at both ages than B 2 B 2 chicks. The results indicate that 4- and 7-wk-old B 5 B 5 chicks had a significantly stronger antibody response to SRBC or BA than B 2 B 2 chicks.
Publication Date
2-1996
Journal Title
Poultry Science
Publisher
Oxford Journals
Digital Object Identifier (DOI)
10.3382/ps.0750203
Scientific Contribution Number
1910
Document Type
Article
Recommended Citation
Dix, Michael C. and Taylor, Robert L. Jr. Differential Antibody Responses in 6.B Major Histocompatibility (B) Complex Congenic Chickens. Poultry Science (1996) 75 (2): 203-207. doi:10.3382/ps.0750203
