Adherence of Salmonella typhimurium to Murine Peritoneal Macrophages Is Mediated by Lipopolysaccharide and Complement Receptors
Abstract
Adherence of Salmonella typhimurium to mouse peritoneal macrophages (Mø) was monitored using a direct microscopic assay and flow cytometry. Competitive binding studies using wild-type lipopolysaccharide and derivatives confirmed a role for this moiety in bacterial adherence. Mø pretreated with 2-deoxy-D-glucose exhibited lower binding activity than did untreated controls, suggesting involvement of either Fc or complement receptors. Pre-exposing Mø to Fc fragments, however, failed to reduce bacterial binding, thus eliminating a role for Fc receptors in this process. Mø pretreated with neutrophil elastase exhibited a diminished ability to bind S. typhimurium, suggesting involvement of complement receptor 1. Monoclonal antibodies M1/70 and M18/2, specific for epitopes on the α and β chains, respectively, of complement receptor 3, also blocked this adherence. In each case we were unable to eliminate completely bacterial adhesion to Mø. Monoclonal antibodies to two additional Mø receptors, Mac-2 and Mac-3, did not block bacterial attachment. These data indicate that multiple mechanisms are involved in the initial adhesion of S. typhimurium to mouse Mø.
Publication Date
6-1997
Journal Title
Zentralblatt für Bakteriologie
Publisher
Elsevier
Digital Object Identifier (DOI)
10.1016/S0934-8840(97)80080-1
Scientific Contribution Number
1941
Document Type
Article
Recommended Citation
al-Bahry, Saif N. and Pistole, Thomas G., "Adherence of Salmonella typhimurium to Murine Peritoneal Macrophages Is Mediated by Lipopolysaccharide and Complement Receptors" (1997). Zentralblatt für Bakteriologie. 260.
https://scholars.unh.edu/nhaes/260
