Effect of ethylene pathway mutations upon expression of the ethylene receptor ETR1 from Arabidopsis
Abstract
The ethylene receptor family of Arabidopsis consists of five members, one of these being ETR1. The effect of ethylene pathway mutations upon expression of ETR1. was examined. For this purpose, ETR1 levels were quantified in mutant backgrounds containing receptor loss-of-function mutations, ethylene-insensitive mutations, and constitutive ethylene response mutations. Ethylene-insensitive mutations of ETR1 resulted in a posttranscriptional increase in levels of the mutant receptor. Treatment of seedlings with silver, which leads to ethylene insensitivity, also resulted in an increase in levels of ETR1. Loss-of-function mutations of ETR1 resulted in both transcriptional and posttranscriptional changes in levels of the receptor. Most other ethylene pathway mutations, including a newly isolated T-DNA insertion mutation in the gene encoding the ethylene receptor ERS1, had relatively minor effects upon the expression of ETR1. Our results indicate that mutations in ETR1 can affect expression at the posttranscriptional level, and suggest that these posttranscriptional changes may contribute to the phenotypes observed in the mutants. Our results also refine the model on how mutations in ethylene receptors are able to confer dominant ethylene insensitivity upon plants.
Publication Date
12-1-2002
Journal Title
Plant Physiology
Publisher
American Society of Plant Physiologists
Digital Object Identifier (DOI)
10.1104/pp.011635
Scientific Contribution Number
2138
Document Type
Article
Rights
© 2002 American Society of Plant Biologists
Recommended Citation
Zhao, Xue-Chu; Qu, Xiang; Mathews, Dennis E.; and Schaller, G. Eric, "Effect of ethylene pathway mutations upon expression of the ethylene receptor ETR1 from Arabidopsis" (2002). Plant Physiology. 173.
https://scholars.unh.edu/nhaes/173