Induced meiotic gynogenesis and sex differentiation in summer flounder (Paralichthys dentatus)

Abstract

Meiogynogenesis and temperature manipulation were used to produce XX male summer flounder broodstock for future production of monosex (all female) populations. Meiogynogens were produced by fertilizing eggs with UV-irradiated (70 mJ/cm(2)) black sea bass sperm and applying 6-minute pressure shocks (58,600 kPa), two min post-fertilization. From 4 females, 132,000 eggs were produced, of which 95.6 +/- 1.8% were viable, 51.0 +/- 13.0% fertilized, and 15.9 +/- 8.3% hatched. Following metamorphosis. meiogynogens and controls were raised under a low temperature regime (12 degrees C gradually increased to 20 degrees C), 21, and 26 degrees C for up to 376 days post hatch (DPH). Female sex differentiation was greater in meiogynogens (62.5%) and control fingerlings (22.6%) raised under a low temperature regime compared to those raised at the higher rearing temperatures: 0% at 21 degrees C, and 0 and 3.9% at 26 degrees C in meiogynogens and controls, respectively. These results suggest that temperature, during the critical phase preceding gonadal development, influences sex differentiation in summer flounder. (C) 2009 Elsevier B.V. All rights reserved.

Publication Date

4-1-2009

Journal Title

Aquaculture

Publisher

Elsevier

Digital Object Identifier (DOI)

10.1016/j.aquaculture.2009.01.005

Scientific Contribution Number

2380

Document Type

Article

Rights

© 2009 Elsevier B.V. All rights reserved.

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