An Updated Outline for Clearing and Staining of Preserved Specimens

Authors

• Sadie E. Smith, University of New Hampshire - Main CampusFollow

Date Completed

Fall 2024

Abstract

This protocol outlines a enhanced clearing and staining procedure for persevered specimens, focusing on the visualization of cartilage and bone in small taxa. With origins from O. Shultze's diaphonization methodology in 1897, the process of clearing and staining improves the clarity and contrast of intricate tissues. It is essential for studies in developmental biology, histology and anatomy. This protocol covers the key steps of the process while detailing the materials, solutions and estimated timeline for each step pertaining to specific taxa. The aim of the protocol is to standardize and provide guidance for procedures in the Biological Sciences department at the University of New Hampshire.

First Advisor

Jessica Bolker

Second Advisor

Janet Anderson

College or School

COLSA

Department or Program

Zoology

Degree Name

Bachelor of Science

Recommended Citation

Smith, Sadie E., "An Updated Outline for Clearing and Staining of Preserved Specimens" (2024). Honors Theses and Capstones. 868.
https://scholars.unh.edu/honors/868