Honors Theses and Capstones
Date Completed
Spring 2023
Abstract
24 Protein Acyl Transferases (PATs) have been identified in the model organism Arabidopsis thaliana. Despite knowing the enzymatic function of these genes (palmitoylation), the specific subcellular pathways, protein targets, and cellular activities of these proteins remain unknown. To investigate the pathways that PAT genes are involved in, deletion mutations were created in several genes and the phenotype of the organisms carrying homozygous mutations was observed. To introduce mutations, the CRISPR/Cas9 system was inserted into the plant’s genome using Agrobacterium tumefaciens to cause deletions in PAT genes of interest. To date, no CRISPR-induced pat mutants have a noticeable phenotype. These results will focus on PAT14; identification of mutants in other PAT genes is underway but will not be presented in detail here.
First Advisor
Estelle Hrabak
College or School
COLSA
Department or Program
Genetics
Degree Name
Bachelor of Science
Recommended Citation
Christopher, Claire E., "Investigation of the Function of Protein Acyl Transferases using CRISPR-Cas9 to Create Null Mutants" (2023). Honors Theses and Capstones. 738.
https://scholars.unh.edu/honors/738