Enzyme-Catalyzed Reactions Using Enzymes Immobilized on Non-spherical Particles
Date of Award
Senior Honors Thesis
College or School
Modern and industrial chemical reactions often utilize enzyme-catalyzed reactions to increase product yields. Given the high cost of enzymes it is desirable to preserve the activity of the enzymes and reuse them several times. In this study, I explored the possibility of immobilizing enzymes on custom-made non-spherical SU-8 microparticles to conduct enzyme-catalyzed reactions in flow reactors. The decomposition of hydrogen peroxide using bovine liver catalase was studied due to its well-understood enzyme kinetics. Soft lithography techniques were used to synthesize prismatic SU-8 microparticles. I will discuss the progress that has been made in immobilizing the enzyme on the particle surface and conducting the enzyme-catalyzed decomposition reaction.
Redfearn, Alexander D., "Enzyme-Catalyzed Reactions Using Enzymes Immobilized on Non-spherical Particles" (2017). Honors Theses and Capstones. 333.