Polyamines and Somatic Embryogenesis in carrot II. The Effects of Cyclohexylammonium phosphate
Abstract
The effects of cyclohexylammonium phosphate (CHAP), a potent inhibitor of spermidine synthase, on Somatic Embryogenesis and cellular polyamine content were studied using carrot (Daucus carota L.) cell cultures. Somatic embryogenesis was only delayed somewhat in the presence of 0.5 mM CHAP. While there was a substantial reduction in the cellular spermidine levels, an increase in putrescine was observed during the 6-day culture period. Spermine was less affected by CHAP than spermidine during the first 24 h, but a significant increase in this polyamine was seen in longer treatments. The activity of arginine decarboxylase was higher in cells treated with CHAP. The effects of CHAP on polyamines and arginine decarboxylase were similar both in the presence and the absence of 2,4-D. The results are in agreement with previous reports on the effects of CHAP on polyamine biosynthesis in plants and animals.
Department
Biological Sciences
Publication Date
2-1-1991
Journal Title
Journal of Plant Physiology
Publisher
Elsevier BV
Digital Object Identifier (DOI)
Document Type
Article
Recommended Citation
A. Jamal Khan, Subhash C. Minocha, Polyamines and Somatic Embryogenesis in carrot II. The Effects of Cyclohexylammonium phosphate, Journal of Plant Physiology, Volume 137, Issue 4, 1991, Pages 446-452, ISSN 0176-1617, https://doi.org/10.1016/S0176-1617(11)80315-9.
Rights
Copyright © 1991 Gustav Fischer Verlag, Stuttgart. Published by Elsevier GmbH All rights reserved.