Pigment and isozyme variation in aspen shoots regenerated from callus culture
Abstract
Pigment as well as isozyme variations were observed among aspen (Populus tremuloides Michx.) plants regenerated from callus cultures. Out of more than 600 plantlets, two chimeric plants (one with green base and two albino shoots and the other with an albino shoot) were produced. Callus derived from albino shoots produced albino as well as chimeric plants when transferred to shoot inducing medium. Isozyme patterns of 119 plants were examined by starch gel electrophoresis. Thirty plants showed variation in shikimic dehydrogenase isozyme and 41 in isocitric dehydrogenase. Variation was also observed in malate dehydrogenase and phosphoglucose isomerase. No variation was seen in 6-phosphogluconate dehydrogenase. Pigment variation was not associated with any isozyme changes.
Department
Biological Sciences
Publication Date
10-1-1990
Journal Title
Plant Cell, Tissue and Organ Culture
Publisher
Springer Science and Business Media LLC
Digital Object Identifier (DOI)
Document Type
Article
Recommended Citation
Noh, EW., Minocha, S.C. Pigment and isozyme variation in aspen shoots regenerated from callus culture. Plant Cell Tiss Organ Cult 23, 39–44 (1990). https://doi.org/10.1007/BF00116087