Shoot regeneration from stem and leaf callus of Eucalyptus tereticornis.
Abstract
Adventitious shoots were obtained from leaf and stem callus of Eucalyptus tereticornis SM. Callus was induced on B5 medium with 0.1 mg/l benzyladenine (BA) and 3 or 5 mg/l naphthalene acetic acid in the dark. Shoot initiation occurred on modified Woody Plant medium (mWP) containing 0.5 mg/l BA, 500 mg/l polyvinylpyrrolidone and 10% (v/v) coconut milk. Multiple shoots were also regenerated directly from hypocotyl segments of 4 to 6 week old seedlings on B5 medium with 0.5 mg/l BA. Regenerated shoots could be rooted with 100% efficiency on mWP medium containing 0.5 mg/l indolebutyric acid and transferred to soil in the greenhouse. Suspension cultures were obtained from the callus using B5 medium with 0.5 mg/l 2,4-dichlorophenoxyacetic acid. Callus clumps grew from less than 1 mm to 4–6 mm in diameter within two weeks on transfer to shoot regeneration medium but failed to form shoots or somatic embryos.
Department
Biological Sciences
Publication Date
11-1-1990
Journal Title
Plant cell reports
Publisher
Springer Science and Business Media LLC
Digital Object Identifier (DOI)
Document Type
Article
Recommended Citation
Subbaiah, M.M., Minocha, S.C. Shoot regeneration from stem and leaf callus of Eucalyptus tereticornis . Plant Cell Reports 9, 370–373 (1990). https://doi.org/10.1007/BF00232401