Effect of Auxin and Abscisic Acid on RNA and Protein Synthesis prior to the First Cell Division in Jerusalem Artichoke Tuber Tissue Cultured in vitro
Abstract
The present paper deals with the effects of auxin (NAA) and abscisic acid on the rates of RNA and protein synthesis at different times during the lag period prior to DNA synthesis in Jerusalem artichoke tuber explants. While an auxin is found to be essential for the induction of DNA synthesis, it is not required for the initiation of RNA or protein synthesis in this tissue. RNA synthesis, as studied by the incorporation of 3H-uridine into TCA insoluble material begins within 2 hrs of excision and incubation in the growth medium. In explants grown in hormonefree medium two peaks of RNA synthesis are seen. The presence of NAA (1 mg/1) causes slight inhibition of RNA synthesis during the first peak while the second peak is slightly delayed to coincide with the initiation of DNA synthesis in the cells. The presence of ABA in the medium further augments NAA effects.
The present paper deals with the effects of auxin (NAA) and abscisic acid on the rates of RNA and protein synthesis at different times during the lag period prior to DNA synthesis in Jerusalem artichoke tuber explants. While an auxin is found to be essential for the induction of DNA synthesis, it is not required for the initiation of RNA or protein synthesis in this tissue. RNA synthesis, as studied by the incorporation of 3H-uridine into TCA insoluble material begins within 2 hrs of excision and incubation in the growth medium. In explants grown in hormonefree medium two peaks of RNA synthesis are seen. The presence of NAA (1 mg/1) causes slight inhibition of RNA synthesis during the first peak while the second peak is slightly delayed to coincide with the initiation of DNA synthesis in the cells. The presence of ABA in the medium further augments NAA effects.
The present paper deals with the effects of auxin (NAA) and abscisic acid on the rates of RNA and protein synthesis at different times during the lag period prior to DNA synthesis in Jerusalem artichoke tuber explants. While an auxin is found to be essential for the induction of DNA synthesis, it is not required for the initiation of RNA or protein synthesis in this tissue. RNA synthesis, as studied by the incorporation of 3H-uridine into TCA insoluble material begins within 2 hrs of excision and incubation in the growth medium. In explants grown in hormonefree medium two peaks of RNA synthesis are seen. The presence of NAA (1 mg/1) causes slight inhibition of RNA synthesis during the first peak while the second peak is slightly delayed to coincide with the initiation of DNA synthesis in the cells. The presence of ABA in the medium further augments NAA effects.
Department
Biological Sciences
Publication Date
1-1-1979
Journal Title
Zeitschrift für Pflanzenphysiologie
Publisher
Elsevier BV
Digital Object Identifier (DOI)
Document Type
Article
Recommended Citation
Subhash C. Minocha, Steven Dibona, Effect of Auxin and Abscisic Acid on RNA and Protein Synthesis prior to the First Cell Division in Jerusalem Artichoke Tuber Tissue Cultured in vitro, Zeitschrift für Pflanzenphysiologie, Volume 92, Issue 4, 1979, Pages 367-374, ISSN 0044-328X, https://doi.org/10.1016/S0044-328X(79)80019-7.
Rights
Copyright © 1979 Gustav Fischer Verlag · Stuttgart. Published by Elsevier GmbH All rights reserved.