Date

4-2017

Project Type

URC Presentation

College or School

COLSA

Class Year

Senior, Junior

Department

Molecular, Cellular and Biomedical Sciences

Major

Biochemistry: Molecular and Cellular Biology; Biomedical Sciences: Medical Laboratory Sciences

Faculty Research Advisor

Paul Tsang

Abstract

In the ovary, growth of a new vascular network, or angiogenesis, is a hallmark of corpus luteum (CL) development. This process requires several angiogenic factors and matrix metalloproteinases (MMPs). Recently, we reported the expression of an angiogenic inducer, Cysteine rich 61-Connective tissue growth factor-Nephroblastoma overexpressed (CCN1), in the bovine CL. However, it is not known how CCN1 is regulated in the CL. Therefore, the goals of the present study were 1) to determine the temporal expression of CCN1, and 2) to determine the regulation by insulin-like growth factor (IGF-1) and luteinizing hormone (LH), during the early (Day 4), mid (Day 8), and late (Day 16) stages of CL development. Following cell culture and quantitative polymerase chain reaction (qPCR), preliminary results showed that CCN1 was rapidly induced within 2 to 4 hours. As such, luteal cells were then treated for 2 hours with IGF-1 (50 ng/mL and 100 ng/mL) and LH (10 ng/mL and 100 ng/mL). Treatment with IGF-1 may increase CCN1 expression in the Day 4 but not in the Day 8 and Day 16 CL, while LH may not have any effect on CCN1 expression in CL cells. Currently, we are evaluating the roles of IGF-1 and LH in MMP expression through analysis of conditioned medium from cell cultures using gelatin zymography. These experiments will be repeated to confirm our observations.

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