Date

5-2017

Project Type

URC Presentation

College or School

COLSA

Class Year

Senior

Department

Molecular, Cellular and Biomedical Sciences

Major

Biology

Faculty Research Advisor

Paul Tsang

Abstract

An evolutionary-conserved family of enzymes known as the matrix metalloproteinases (MMPs) play major roles in tissue remodeling. While much is known about MMPs in mammalian vertebrates, their presence and function in non-mammalian vertebrates is not well studied. In teleosts, MMPs have been shown to degrade skeletal muscle and thus, may affect the quality of fillet at market. Standard commercial fishing practices can be stressful, which could also adversely affect fillet quality. Interestingly we observed that MMPs are expressed in skeletal muscle of the spiny dogfish, Squalus acanthias. Therefore, we hypothesized that skeletal muscle MMP expression in dogfish is correlated to capture method. Skeletal muscles were obtained from dogfish caught under high stress (24-hr tow) and low stress (hook and line) conditions. Dogfish skeletal muscle proteins were extracted and subjected to gelatin zymography to identify MMPs. Among them, a few were deferentially expressed in dogfish caught using high and low stress methods. These results may have implications for commercial fishing practices that ultimately provide a high-quality product to consumers.

Rights

Document References (1) Detection of Functional Matrix Metalloproteinases by Zymography Xueyou Hu1, Christine Beeton1 1Department of Molecular Physiology and Biophysics, Baylor College of Medicine (2) Kubota, Satoshi, et al. "Occurrence of two distinct types of tissue inhibitor of metalloproteinases-2 in teleost fish." Biochimica et Biophysica Acta (BBA)-Gene Structure and Expression 1629.1 (2003): 102-108. (3) Nagase, Hideaki, Robert Visse, and Gillian Murphy. "Structure and function of matrix metalloproteinases and TIMPs." Cardiovascular research 69.3 (2006): 562-573. Image References https://ghsmp.org/fishery-economics-sustainable-fish-consumption-and-banning-industrial-fishing-article/ (fishing trawler) http://www.greenpeace.org/international/Global/international/planet-2/image/2008/5/hooks-and-lines.jpg hook and line http://www.greenpeace.org/international/Global/international/planet-2/image/2008/5/beam-trawling.jpg bottom trawl http://2014.igem.org/Team:WPI-Worcester/Overview (Plasma pre-edit) https://cellularphysiology.wikispaces.com/Gel+Electrophoresis gel electrophoresis Gong, Yixuan, Uma D. Chippada-Venkata, and William K. Oh. "Roles of matrix metalloproteinases and their natural inhibitors in prostate cancer progression." Cancers 6.3 (2014): 1298-1327. MMP Image

Download

Share

COinS