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Abstract
The conserved and structured elements in viral RNA genomes interact with proteins to regulate various events in the viral life cycle and have become key targets for developing novel therapeutic approaches. We probe physical interactions between lab-evolved proteins and a viral RNA element from the HIV-1 genome. Specifically, we study the role of an arginine-rich loop in recognition of designed proteins by the viral RNA element. We report free energy calculations to quantitatively estimate the protein/RNA binding energetics, focusing on the mutations of arginine residues involved in recognition of the major groove of RNA by proteins.
Publication Date
10-29-2021
Publisher
Royal Society of Chemistry
Journal Title
Physical Chemistry Chemical Physics
Digital Object Identifier (DOI)
Document Type
Article
Recommended Citation
Amit Kumar and Harish Vashisth. Conformational dynamics and energetics of viral RNA recognition by lab-evolved proteins, Phys. Chem. Chem. Phys., 2021, 23, 24773-24779, https://doi.org/10.1039/D1CP03822B
Comments
This is an Open Access article published by Royal Society of Chemistry in Physical Chemistry Chemical Physics in 2021, available online: https://dx.doi.org/10.1039/D1CP03822B