Honors Theses and Capstones
The relationship between alpha-defensin 1 and gut microbiome composition in a population of Bhutanese refugee adults with a high prevalence of type 2 diabetes
Date of Award
Senior Honors Thesis
College or School
Program or Major
Biomedical Sciences: Medical & Veterinary Sciences
Bachelor of Science
Dr. Maria Carlota Dao
The New Hampshire Bhutanese refugee population is disproportionately affected by obesity and metabolic disorders such as type 2 diabetes. The gut microbiome is important for overall human health, with high microbial richness and diversity being associated with reduced chronic inflammation and type 2 diabetes risk. Microbial richness and diversity have been shown to decrease in United States (U.S.) immigrant populations, but have not been measured in refugee populations. One of the anti-inflammatory functions of the gut microbiome is related to the production of short-chain fatty acids (SCFAs), which regulate pro-inflammatory cytokine production. Alpha-defensin 1 is an antimicrobial peptide that is a major player in the innate immune response to inflammation. This peptide is expressed at an increased rate when inflammation is present in the body, but is known to have decreased expression in individuals with obesity and type 2 diabetes (T2D) compared to individuals without metabolic disorder. The purpose of this thesis was to analyze the potential relationship between alpha-defensin 1 and gut microbiome species richness and diversity measures (Shannon-Wiener species diversity index, Inverse Simpson’s diversity index, and Fisher’s alpha diversity index) to see if T2D status affected the relationship between these variables. Additionally, the correlation between interleukin 6 (IL-6) and gut microbiome species richness and diversity was analyzed due to a previous finding that IL-6 and alpha-defensin 1 had a significant positive correlation. The fecal and plasma samples used to obtain the data in this project were collected from a volunteer population of 50 Bhutanese refugee adults who were residing in New Hampshire at the time of sample collection.
Drake, Daisha Alana, "The relationship between alpha-defensin 1 and gut microbiome composition in a population of Bhutanese refugee adults with a high prevalence of type 2 diabetes" (2023). Honors Theses and Capstones. 743.