Analytical Ultracentrifugation: A Powerful ‘New’ Technology
Abstract
Analytical ultracentrifugation (AUC) is a powerful means of characterizing the solution behavior of molecules. Sedimentation velocity analysis, the preferred AUC technique for characterizing complex systems, has higher resolution, broader applicable range and fewer solute/solvent limitations than gel-permeation chromatography. The technique is simple to perform and should become a mainstay for target identification, target validation, lead optimization, formulation in drug development and QA/QC. Recent studies have used AUC to characterize the binding stoichiometry and binding sites of an anti-tumor agent; of a hemoglobin-stabilizing protein, and of a fibril growth inhibitor, and to assess the causes of protein aggregation. The recent addition of fluorescence to the existing absorbance and interference detectors dramatically extends the flexibility of analytical ultracentrifugation.
Department
Molecular, Cellular and Biomedical Sciences
Publication Date
12-1-2004
Journal Title
Drug Discovery Today: Technologies
Publisher
Elsevier
Digital Object Identifier (DOI)
Document Type
Book Chapter
Recommended Citation
Laue, T.M. (2004) "Analytical Ultracentrifugation: A Powerful ‘New’ Technology" in Drug Discovery Today: Technologies, 1:309-315.