Polyamine Biosynthetic Enzymes and the Effect of their Inhibition on the Growth of Some Phytopathogenic Fungi
Abstract
Studies were conducted on the distribution of two polyamine biosynthetic enzymes, or-nithine decarboxylase (ODC) and arginine decarboxylase (ADC), and the effect of their inhibitors on growth and polyamine biosynthesis in four phytopathogenic fungi, namely, Helminthosporium maydis, H. carbonum, Fusarium oxysporum f. sp. lycopersici and Ceratocystis ulmi. Three species had high level of ODC as compared to ADC activity; in C. ulmi on the other hand, ADC was predominant with very little or no ODC activity. DL-α-difluoromethylornithine (DFMO) significantly inhibited ODC activity in all species in vitro with little effect on ADC activity. ADC in all cases was inhibited by DL-α-difluoromethylarginine (DFMA) but not by DFMO. Mycelial growth of all fungi was inhibited by 1 to 5 mM concentrations of either DFMO or DFMA within two days except in H. maydis which remained unaffected even by the highest concentration (5 mM) of DFMA. In general, the inhibition was more pronounced with DFMO as compared to DFMA. Putrescine completely reversed the inhibitory effects of DFMO and DFMA in all species. Among the polyamines, spermidine was predominant in all fungi. The cellular concentrations of putrescine and spermidine were considerably lower in the presence of either of the inhibitors while spermine levels were higher than the control.
Department
Biological Sciences
Publication Date
7-1-1989
Journal Title
Plant and Cell Physiology
Publisher
Oxford University Press (OUP)
Digital Object Identifier (DOI)
Document Type
Article
Recommended Citation
Akhtar J. Khan, Subhash C. Minocha, Polyamine Biosynthetic Enzymes and the Effect of their Inhibition on the Growth of Some Phytopathogenic Fungi, Plant and Cell Physiology, Volume 30, Issue 5, July 1989, Pages 655–663, https://doi.org/10.1093/oxfordjournals.pcp.a077790
Rights
© 1989 The Japanese Society of Plant Physiologists (JSPP)