Effects of n-Sodium Butyrate on Cell Division in Jerusalem Artichoke (Helianthus tuberoses L.) Tuber Explants Cultured in vitro
Abstract
The effects of n-sodium butyrate on cell division, DNA synthesis, RNA synthesis, and protein synthesis in synchronously dividing explant cultures of Jerusalem artichoke (Helianthus tuberoses L.) tuber tissue were investigated. While 0.1 mM butyrate had no effect on auxin-induced cell division in the explants during the 7 day culture period, 1.0 and 5.0 mM butyrate caused a complete inhibition of DNA synthesis. Inhibition of DNA synthesis occurred regardless of when during the lag period butyrate was added to the cultures. Addition of butyrate after the initiation of S phase resulted in a rapid decline in the rates of DNA synthesis to background levels. This effect was completely reversed upon removal of butyrate. The response to butyrate was apparently a reflection of the effect on DNA synthesis rather than on the uptake of 3H-thymidine.
The effects of butyrate on RNA and protein synthesis were more ambiguous. Although butyrate substantially inhibited the incorporation of 3H-uridine and 3H-leucine into RNA and proteins respectively, it also caused reductions in the uptake of these precursors. No significant effect of butyrate on the acetylation of histones was seen in this tissue.
Department
Biological Sciences
Publication Date
6-1-1988
Journal Title
Journal of Plant Physiology
Publisher
Elsevier BV
Digital Object Identifier (DOI)
Document Type
Article
Recommended Citation
Thomas J. Pedersen, Subhash C. Minocha, Effects of n-Sodium Butyrate on Cell Division in Jerusalem Artichoke (Helianthus tuberoses L.) Tuber Explants Cultured in vitro, Journal of Plant Physiology, Volume 132, Issue 5, 1988, Pages 623-630, ISSN 0176-1617, https://doi.org/10.1016/S0176-1617(88)80266-9.
Rights
Copyright © 1988 Gustav Fischer Verlag, Stuttgart. Published by Elsevier GmbH All rights reserved.