Kinetics of Phenylalanine Ammonia-Lyase and the Effect of L-α-aminooxy-β-phenylpropionic Acid on Enzyme Activity and Radicle Growth in Germinating Lettuce Seeds
Abstract
The effects of different concentrations of L-α-aminooxy-β-phenyIpropionic acid (AOPP), an analog of L-phenylalanine, on the activity of phenylalanine ammonia-lyase (PAL, EC 4.3.1.5) and the growth of radicles in 24 h old germinating lettuce (Lactuca saliva L.) seeds were investigated. AOPP causes a significant inhibition of PAL activity in the seeds (85% inhibition at 104 M). It also causes a stimulation of radicle growth at that concentration. The results show that the inhibition of PAL by AOPP may be due to an irreversible binding of the inhibitor to the enzyme leading to its inactivation. AOPP also inhibits ethylene biosynthesis in germinating lettuce seeds which could probably explain the stimulation of radicle growth in these seeds.
The enzyme shows typical Michaelis-Menten kinetics. The Km for L-phenylalanine is 4.2 × 105 M. The enzyme does not show any tyrosine ammonia-lyase activity. Various substrate analogs such as D-phenylalanine, p-fluorophenylalanine, β-phenyllactic acid, tryptophan and the product of the enzyme reaction, trans-cinnamic acid, inhibit the enzyme competitively. A number of intermediates and endproducts of the phenylpropanpid pathway, except chlorogenic acid, do not show any inhibition.
Department
Biological Sciences
Publication Date
12-1-1986
Journal Title
Plant and Cell Physiology
Publisher
Oxford University Press (OUP)
Digital Object Identifier (DOI)
Document Type
Article
Recommended Citation
G. Jose Kudakasseril, Subhash C. Minocha, Kinetics of Phenylalanine Ammonia-Lyase and the Effect of L-α-aminooxy-β-phenylpropionic Acid on Enzyme Activity and Radicle Growth in Germinating Lettuce Seeds, Plant and Cell Physiology, Volume 27, Issue 8, December 1986, Pages 1499–1506, https://doi.org/10.1093/oxfordjournals.pcp.a077250
Rights
© 1986 The Japanese Society of Plant Physiologists