Regulation of Phenylalanine Ammonia-lyase in Germinating Lettuce Seeds (Lactuca sativa L. cv. Grand Rapids): Effects of Abscisic Acid and Water Stress*
Abstract
Abscisic acid (ABA) and polyethylene glycol (PEG, 35%) completely inhibited germination of Grand Rapids lettuce seeds in light. A parallel inhibition of the activity of L-phenylalanine ammonia-lyase (PAL) was also observed under these conditions. Effects of ABA were not reversed by GA3 and only slightly reversed by benzyladenine. Seeds germinated in water showed a steady increase in PAL activity during a 20 h period. If seeds germinated in water were transferred to 0.02 mM ABA, the activity of PAL in these seeds significantly declined during the next 24 h. Transfer of germinated seeds to PEG resulted in a much faster loss of PAL activity during this period. The disappearance of PAL activity on transfer to ABA and PEG occurred with calculated half-lives of 11 and 3 h, respectively. The loss of PAL caused by PEG was readily reversible on removal of the osmoticum.
Department
Biological Sciences
Publication Date
4-1-1983
Journal Title
Zeitschrift für Pflanzenphysiologie
Publisher
Elsevier BV
Digital Object Identifier (DOI)
Document Type
Article
Recommended Citation
Richard J. Daines, Subhash C. Minocha, Regulation of Phenylalanine Ammonia-lyase in Germinating Lettuce Seeds (Lactuca sativa L. cv. Grand Rapids): Effects of Abscisic Acid and Water Stress, Zeitschrift für Pflanzenphysiologie, Volume 110, Issue 1, 1983, Pages 69-76, ISSN 0044-328X, https://doi.org/10.1016/S0044-328X(83)80217-7.
Rights
Copyright © 1983 Gustav Fischer Verlag, Stuttgart. Published by Elsevier GmbH All rights reserved.