Callus and adventitious shoot formation in excised embryos of white pine (Pinus strobus)
Abstract
Excised embryos from 2- to 3-year-old seeds of white pine (Pinus strobus) were grown on Murashige and Skoog medium supplemented with several concentrations of different plant hormones. In the basal medium the embryos developed into normal seedlings. At 1–2% sucrose concentration in the medium, the primary root of the embryo did not grow well, while at 3–6% sucrose concentration normal growth of the primary root was observed in most of the embryos.Gibberellic acid at a wide range of concentrations (0.01–20.0 mg/L) had little effect on the growth of the seedling shoot while growth of the primary root was generally suppressed. In the presence of 0.01–2 mg/L of NAA, 2,4-D, or IBA, the embryos proliferated to form friable green callus. Callus formation was further enhanced by a cytokinin (0.1-5 mg/L). IBA (1–5 mg/L) in some embryos induced the formation of adventitious shoots from the hypocotyl region. Similarly, TIBA (0.5–1.0 mg/L) induced the formation of adventitious shoots but they were formed at the tips ...
Department
Biological Sciences
Publication Date
2-1-1980
Journal Title
Canadian Journal of Botany
Publisher
Canadian Science Publishing
Digital Object Identifier (DOI)
Document Type
Article
Recommended Citation
Subhash C. Minocha. 1980. Callus and adventitious shoot formation in excised embryos of white pine (Pinus strobus). Canadian Journal of Botany. 58(3): 366-370. https://doi.org/10.1139/b80-037