Abscisic Acid Promotion of Cell Division and DNA Synthesis in Jerusalem Artichoke Tuber Tissue Cultured in vitro
Abstract
When excised tuber explants of Jerusalem artichoke (Helianthus tuberoses L.) are cultured in vitro, an auxin is found to be essential for the induction of growth by celb division. Abscisic acid further stimulates this growth when used in combination with the auxin. The stimulation of cell division is detectable within 48 hrs of incubation and lasts up to 2-3 weeks of culture. Further studies reveal that ABA has no effect on the timing of onset or the total duration of DNA synthesis phase («S» phase) of the cell cycle, but actually induces a higher percentage of cells in the explant to undergo DNA replication and cell division. While only 30-40% of the total cell population divide in presence of 1 mg/l NAA, this proportion is increased to approximately 65% by the presence of 2 mg/l ABA in addition to NAA. A 50-100% increase in the rate of DNA synthesis (as measured by the incorporation of 3H-thymidine into TCA-insoluble material) prior to first cell division is observed in the explants in presence of ABA. This increase is visible throughout the duration of S phase. The promotory effect of ABA is visible over a wide range of concentrations (0.1-10 mg/1). Abscisic acid does not significantly affect the uptake of 3H-thymidine by the tissue. Benzylamino-purine (5 mg/1) reverses the stimulatory effect of ABA.
Storage of tubers in the dark at 4°C for more than 6-8 weeks causes a delay in the initiation of DNA synthesis in response to auxin. A slight increase in the total length of the S phase is also observed in old tubers. However, there is no noticeable change in the response of the tissue to ABA as a result of storage.
Department
Biological Sciences
Publication Date
1-1-1979
Journal Title
Zeitschrift für Pflanzenphysiologie
Publisher
Elsevier BV
Digital Object Identifier (DOI)
Document Type
Article
Recommended Citation
Subhash C. Minocha, Abscisic Acid Promotion of Cell Division and DNA Synthesis in Jerusalem Artichoke Tuber Tissue Cultured in vitro, Zeitschrift für Pflanzenphysiologie, Volume 92, Issue 4, 1979, Pages 327-339, ISSN 0044-328X, https://doi.org/10.1016/S0044-328X(79)80015-X.
Rights
Copyright © 1979 Gustav Fischer Verlag · Stuttgart. Published by Elsevier GmbH All rights reserved.