https://dx.doi.org/10.2307/2441186">
 

Nutritional and Morphogenetic Investigations on Callus Cultures of Neomammillaria profilera Miller (Cactaceae)

Abstract

Neomammillaria prolifera (Cactaceae), when grown on Murashige and Skoog's medium supplemented with fresh coconut milk, showed very little growth. Various concentrations and combinations of growth regulators which did not cause callusing had no apparent effect on the normal growth rates of intact plants. Healthy green calli obtained on a 2,4-D and kinetin-containing medium exhibited extremely fast growth and very specific growth requirements. Relatively high amounts of 2,4-D (10–20 mg/liter), kinetin (1–2 mg/liter), and coconut milk (20–60%) were required at all times for continued proliferation of callus on subculturing. Moreover, the callus was very tolerant to extremely high concentrations of other growth regulators (IAA, NAA, IBA, and GA up to 100 mg/liter) in the presence of 2,4-D and coconut milk. These substances could not replace 2,4-D for callusing or continued growth of callus. It was not possible to establish root cultures or to induce callusing of roots. Attempts to induce differentiation in callus were unsuccessful, except for sporadic root initiation in some cultures. A comparison of these results with similar studies on other succulents demonstrates some basic physiological similarities among this group of plants.

Department

Biological Sciences

Publication Date

2-1-1974

Journal Title

American Journal of Botany

Publisher

Wiley

Digital Object Identifier (DOI)

https://dx.doi.org/10.2307/2441186

Document Type

Article

Rights

© 1974 Botanical Society of America

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