Nutritional and Morphogenetic Investigations on Callus Cultures of Neomammillaria profilera Miller (Cactaceae)
Abstract
Neomammillaria prolifera (Cactaceae), when grown on Murashige and Skoog's medium supplemented with fresh coconut milk, showed very little growth. Various concentrations and combinations of growth regulators which did not cause callusing had no apparent effect on the normal growth rates of intact plants. Healthy green calli obtained on a 2,4-D and kinetin-containing medium exhibited extremely fast growth and very specific growth requirements. Relatively high amounts of 2,4-D (10–20 mg/liter), kinetin (1–2 mg/liter), and coconut milk (20–60%) were required at all times for continued proliferation of callus on subculturing. Moreover, the callus was very tolerant to extremely high concentrations of other growth regulators (IAA, NAA, IBA, and GA up to 100 mg/liter) in the presence of 2,4-D and coconut milk. These substances could not replace 2,4-D for callusing or continued growth of callus. It was not possible to establish root cultures or to induce callusing of roots. Attempts to induce differentiation in callus were unsuccessful, except for sporadic root initiation in some cultures. A comparison of these results with similar studies on other succulents demonstrates some basic physiological similarities among this group of plants.
Department
Biological Sciences
Publication Date
2-1-1974
Journal Title
American Journal of Botany
Publisher
Wiley
Digital Object Identifier (DOI)
Document Type
Article
Recommended Citation
Minocha, S.C. and Mehra, P.N. (1974), NUTRITIONAL AND MORPHOGENETIC INVESTIGATIONS ON CALLUS CULTURES OF NEOMAMMILLARIA PROFILERA MILLER (CACTACEAE). American Journal of Botany, 61: 168-173. https://doi.org/10.1002/j.1537-2197.1974.tb06042.x
Rights
© 1974 Botanical Society of America