Date of Award
Spring 2009
Abstract
Genetic variation within the diploid Daphnia pulex genome was examined using a high quality de novo assembly and shotgun reads from two distinct D. pulex clones. Patterns of variation and divergence at single nucleotides were examined in physical and functional regions of the genome using comparative assembly output and available annotations. Additionally, mitochondrial genomes of the same D. pulex clones were assembled and compared for patterns of divergence, and substitutional biases. Intron presence/absence polymorphisms were identified computationally and verified experimentally. Finally, gene duplicate demographics were examined for patterns of divergence and estimates of gene birth rates.
Document Type
Dissertation
First Advisor
W Kelley Thomas
Department or Program
Genetics
Degree Name
Doctor of Philosophy
Recommended Citation
Tucker, Abraham Eaton, "Genetic variation within the Daphnia pulex genome" (2009). Doctoral Dissertations. 490.
https://scholars.unh.edu/dissertation/490