Cellular adaptations in fat tissue of exercise-trained miniature swine: role of excess energy intake

Abstract

This study examined the influence of energy expenditure and energy intake on cellular mechanisms regulating adipose tissue metabolism.(1) Twenty-four swine were assigned to restricted-fed sedentary, restricted-fed exercise-trained, full-fed sedentary, or full-fed exercise-trained groups. After 3 mo of treatment, adipocytes were isolated and adipocyte size, adenosine A(1) receptor characteristics, and lipolytic sensitivity were measured. Swine were infused with epinephrine during which adipose tissue extracellular adenosine, plasma fatty acids, and plasma glycerol were measured. Results revealed that adipocytes isolated from restricted-fed exercised swine had a smaller diameter, a lower number of A(1) receptors, and a greater sensitivity to lipolytic stimulation, compared with adipocytes from full-fed exercised swine. Extracellular adenosine levels were transiently increased on infusion of epinephrine in adipose tissue of restricted-fed exercised but not full-fed exercised swine. These-results suggest a role for adenosine in explaining the discrepancy between in vitro and in vivo lipolysis findings and underscore the notion that excess energy intake dampens the lipolytic sensitivity of adipocytes to beta-agonists and adenosine, even if accompanied by exercise training.

Publication Date

3-1-2000

Journal Title

Journal of applied physiology

Publisher

American Physiological Society

Scientific Contribution Number

2036

Document Type

Article

Rights

Copyright © 2000 the American Physiological Society

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