Honors Theses and Capstones

Date Completed

Spring 2025

Abstract

Lyophilization, or freeze-drying, is an important preservation method for protein-based products, such as protein pharmaceuticals. However, the process introduces both freezing and drying stresses that can harm proteins. Here, we have studied the efficacy of AnAFP, a dehydrin-like antifreeze protein isolated from the broadleaf desert shrub Ammopiptanthus nanus, in reducing enzyme damage during freeze-drying, or lyophilization. We have shown that AnAFP can protect the model enzyme lactate dehydrogenase (LDH) from both freezing and dehydration damages during lyophilization with similar or better efficacy than 10% w/v sucrose and superior to that of another known antifreeze protein.

First Advisor

Krisztina Varga

College or School

COLSA

Department or Program

Biochemistry, Molecular and Cellular Biology

Degree Name

Bachelor of Science

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Included in

Biochemistry Commons

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