Date of Award

Winter 2010

Project Type

Thesis

Program or Major

Genetics

Degree Name

Master of Science

First Advisor

Estelle M Hrabak

Abstract

This study seeks to understand the effects of loss of palmitoylation catalyzed by PAT14 on the phenotype of Arabidopsis thaliana. In this project, I focused on two T-DNA insertion mutants of the PAT14 gene in Arabidopsis thaliana: pat14-1 and pat14-2. PAT14 is one of 23 PAT genes encoding palmitoyltransferase (PAT) proteins in Arabidopsis thaliana. PAT proteins are responsible for palmitoylation, one of several cellular mechanisms to modify proteins by lipidation. The purpose of this research was to describe the effect of T-DNA insertion mutations in the PAT14 gene on the phenotype of Arabidopsis thaliana plants. Characterization of palmitoyltransferase mutants of Arabidopsis thaliana indicated that lack of palmitoylation by PAT14 has a role in plant growth, affecting leaves and roots, seed production and germination.

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