Date of Award
Program or Major
Master of Science
Thomas M Davis
A unique pentaploid population of Fragaria (strawberry) was developed to gain insight into octoploid genome composition and allele transmission patterns. This population was produced by crossing representatives of two divergent subspecies of octoploid F. virginiana and then by crossing an octoploid hybrid plant with diploid F. vesca. To enable examination of allele transmission patterns, the intergenic region of the gRGA1-Subtilase gene pair locus was amplified, and PCR products were cloned, sequenced, and compared to define the allele composition of the hybrid octoploid. The sequencing data revealed three distinct major haplotypes and additional subtypes. The pentaploids were genotyped utilizing the FEL-CAPS method. From these results, it was possible to detect disomic inheritance of an allele pair within one major haplotype. This result establishes the potential value of the pentaploid population as a resource for analysis of octoploid allele transmission patterns in strawberry.
Poulsen, Elizabeth, "Using pentaploids as tools for studying genomic composition and allele transmission patterns in octoploid Fragaria" (2010). Master's Theses and Capstones. 585.