Date of Award

Winter 2006

Project Type


Program or Major

Materials Science

Degree Name

Master of Science

First Advisor

Yvon Durant


A series of non-covalent Molecularly Imprinted Polymers (MIPs) were prepared using caffeine and guanosine separately as template molecules. A wide variety of MIPs were prepared by varying a number of formulation parameters, including the template, recognition monomer, crosslinking agent and porogen types and concentrations. The specificity and selectivity of the MIPs were determined by rebinding studies in an aqueous medium using HPLC detection and the results were supported by gas adsorption experiments. Rebinding conditions, such as the pH and MIP concentration were also varied to determine the best conditions for analyte recognition. By using variety of polymerization conditions the particle size of the MIPs ranged from 500 nm to 200 mum. Their rebinding characteristics were analyzed.

An optimized formulation with suitable rebinding conditions was determined. By balancing the binding strength and specific site density the highest recognition performance was identified.