Microwave-Assisted Glycosaminoglycans Labeling Using 1-phenyl-3-methyl-5-pyrazolone Followed by MALDI-TOF-MS Analysis

Date

4-2017

Project Type

URC Presentation

College or School

COLSA

Class Year

Junior

Department

Biological Sciences

Major

Biomedical Science: Medical Microbiology

Faculty Research Advisor

Qing Guo

Second Faculty Research Advisor

Vernon Reinhold

Abstract

Glycoaminoglycans (GAGs) are linear polyionic polymers that participate in a host of critically important biological processes such as blood anticoagulation, pathogen infection, cell differentiation, growth, migration and inflammation, etc. Analysis of these glycans holds substantial significance in the field of medicine as knowledge of protein function and structure are essential to breaking down barriers to disease causation and function.

The objective of this experiment is to determine which temperature and time protocol would be best used for 1-phenyl-3-methyl-5-pyrazolone (PMP) labeling of GAGs. Glycosaminoglycan disaccharides used in this experimentation were treated to three different temperatures (70°C, 100°C, 150°C) and four different time frames (30 minutes, 1 hour, 2 hours, 3 hours) in microwave radiation. After reaction, the PMP-labeled glycans were analyzed by Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass spectrometry (MALDI-TOF-MS).

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