Title

Microvascular endothelial cells of the bovine corpus luteum: A comparative examination of the estrous cycle and pregnancy

Abstract

Endothelial cells derived from the corpus luteum (CLENDOs) exhibit diverse characteristics presumably serving their wide-ranging roles in luteal function and fate. Here, several attributes of CLENDOs derived from cows at midcycle (days 9-12 of the estrous cycle) were compared with CLENDOs from early pregnancy (day 60 of pregnancy). Flow cytometric analysis of cells fluorescently-tagged with the lectins Bandeiraea simplicifolia (BS-1) and Concanavalin A (ConA) indicated that CLENDOs of midcycle CL do not differ from those of pregnancy. Mean fluorescence intensity for BS-1 was 15 +/- 1 and 23 +/- 7 fluorescent units for midcycle CLENDOs and CLENDOs of pregnancy, respectively (P > 0.05). For ConA, mean fluorescence was 25 2 and 26 1 fluorescent units, respectively (P > 0.05). The CLENDCs were also exposed to cytokines to assess differences in activation of nuclear factor kappa B signaling (NF-kappa B), induction of the transcription factor interferon regulatory factor 1 (IRF1), cytokine production, and cytokine-induced cell death. In response to TNF, for instance, both types of CLENDOs exhibited a rapid, 5-fold decrease in NF-kappa B inhibitor alpha (NFKBIA) protein expression (P 0.05). Similarly, both types of CLENDCs produced tumor necrosis factor alpha and chemokine ligand 2 in response to IFNG stimulation (P 0.05). Lastly, extended exposure of CLENDOs of midcycle CL to cytokines induced cell death (similar to 50% cell death vs. control) similar to the incidence of cell death seen previously in CLENDOs of early pregnancy. The results indicate that several physical and functional characteristics of CLENDOs of midcycle CL are retained through early pregnancy, including lectin-binding properties, sensitivity to cytokines, and the activation of cytokine-initiated intracellular signals.

Publication Date

6-2008

Journal Title

Journal of Reproduction and Development

Publisher

Society for Reproduction and Development

Digital Object Identifier (DOI)

10.1262/jrd.19182

Document Type

Article

Rights

Copyright © 2008 Society for Reproduction and Development