Microwave-Assisted Glycosaminoglycans Labeling Using 1-phenyl-3-methyl-5-pyrazolone Followed by MALDI-TOF-MS Analysis
College or School
Biomedical Science: Medical Microbiology
Faculty Research Advisor
Second Faculty Research Advisor
Glycoaminoglycans (GAGs) are linear polyionic polymers that participate in a host of critically important biological processes such as blood anticoagulation, pathogen infection, cell differentiation, growth, migration and inflammation, etc. Analysis of these glycans holds substantial significance in the field of medicine as knowledge of protein function and structure are essential to breaking down barriers to disease causation and function.
The objective of this experiment is to determine which temperature and time protocol would be best used for 1-phenyl-3-methyl-5-pyrazolone (PMP) labeling of GAGs. Glycosaminoglycan disaccharides used in this experimentation were treated to three different temperatures (70°C, 100°C, 150°C) and four different time frames (30 minutes, 1 hour, 2 hours, 3 hours) in microwave radiation. After reaction, the PMP-labeled glycans were analyzed by Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass spectrometry (MALDI-TOF-MS).
Guerrero, Avianna, "Microwave-Assisted Glycosaminoglycans Labeling Using 1-phenyl-3-methyl-5-pyrazolone Followed by MALDI-TOF-MS Analysis" (2017). Undergraduate Research Conference (URC) Student Presentations. 391.