Date of Award
Program or Major
Master of Science
Palligarnai T Vasudevan
In this thesis, the effect of organic solvent-cosolvent system, temperature, methanol concentration, water content and used enzyme washing method on transesterification of waste vegetable oil catalyzed by Thermomyces lanuginosus lipase immobilized on zeolite ZSM-5 was investigated. Four organic solvents, one co-solvent, three reaction temperatures, three methanol addition methods, four different water content and two used-enzyme washing methods were assessed. Optimal conditions were obtained with n-hexane as solvent, 5 vol.% tert-butanol as co-solvent, 25°C reaction temperature, batch addition of 3 equivalent molar methanol, water content equivalent to 7.5% by weight, and used-enzyme washing method B. It was also confirmed that the transesterification reaction catalyzed by used Thermomyces lanuginosus lipase immobilized on zeolite ZSM-5 was limited by the concentration of acyl acceptor, and that active water loss was related to enzyme activity lost after every reaction cycle.
Deng, Ye, "Transesterification of waste vegetable oil by Thermomyces lanuginosus lipase immobilized on zeolite ZSM-5" (2012). Master's Theses and Capstones. 734.