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Although many studies primarily discuss gay men and lesbian women, very few have focused on the bisexual population. The few previous studies conducted about bisexuality show that many people have negative attitudes toward bisexuality. This study examines the effects of gender and sexual orientation on attitudes toward bisexuality. 378 students at the University of New Hampshire took surveys asking whether they believed bisexuality was a legitimate sexual orientation. The data showed no statistically significant differences in attitudes between men, women, or other sexual orientations. However, the majority of students in general believed that bisexuality is a legitimate sexual orientation. Another large portion had “neutral” attitudes, suggesting a lack of knowledge about the bisexual population, demonstrating a reluctance to formulate opinions about them. Further research on larger and more diverse samples is still necessary to more accurately measure attitudes toward bisexuality.