High-performance liquid chromatographic method for the determination of dansyl-polyammines
This paper describes a fast reliable, and a sensitive technique for the separation and quantification of dansylated polyamines by high-performance liquid chromatography. Using a small 33 × 4.6 mm I.D., 3 μm particle size, C18 reversed-phase cartridge column and a linear gradient of acetonitrile—heptanesulfonate (10 mM, Ph 3.4), at a flow-rate of 2.5 ml/min, the retention time for different polyamines was: N8-acetyl-spermidine, 1.79 min; N1-acetylspermidine, 1.82 min;putrescine, 2.26 min; cadaverine, 2.43 min; heptanediamine, 2.83 min; spermidine, 3.42 min; and spermine, 4.41 min. With an additional column regeneration time of 3—4 min, the complete cycle per sample took less than 8 min at room temperture. Using a fluorescence detector, the lower limit of detection was less than 1 pmol per 6 μl injection volume. The fluorescence response was linear up to 200 pmol per 6 μl for each polyamine. The method is suitable for separation of polyamines from animal, plant and fungal sources.
Journal of Chromatography A
Digital Object Identifier (DOI)
Scientific Contribution Number
Minocha, Subhash C., Minocha, R., and Robie, Cheryl A. High-performance liquid chromatographic method for the determination of dansyl-polyammines. Journal of Chromatography A, Volume 511, 1990, Pages 177-183, ISSN 0021-9673, http://dx.doi.org/10.1016/S0021-9673(01)93283-2.