Characterization of guanylate cyclase in squid photoreceptors.
Light causes a rapid, 1.7-fold increase in cyclic GMP concentration in intact squid retinas (Johnson et al. (1986)). To determine whether light-induced changes in cyclic GMP concentration result from activation of guanylate cyclase, we have studied the regulation of guanylate cyclase activity in squid (Loligo pealei) photoreceptors. The enzyme is membrane-associated and activity is enhanced by the detergents Triton X-100 or digitonin. The enzyme requires divalent cations, Mn2+ being preferred over Mg2+. The dependence of enzyme activity on the MnGTP concentration deviates from simple Michaelis-Menten kinetics. Under conditions where a light-induced binding of GTP to the guanine nucleotide regulatory protein can be observed, no light-induced change in guanylate cyclase could be detected.
Molecular, Cellular and Biomedical Sciences
Digital Object Identifier (DOI)
Phyllis R. Robinson and Richard H. Cote (1989). Characterization of guanylate cyclase in squid photoreceptors. Visual Neuroscience, 3, pp 1-7. doi:10.1017/S0952523800012451.