https://dx.doi.org/10.1073/pnas.92.23.10777">
 

Abstract

Cytoplasmic free-Ca2+ levels in Escherichia coli were measured by use of the fluorescent Ca(2+)-indicator dye fura-2. Chemotactically wild-type E. coli regulated cytoplasmic free Ca2+ at approximately 100 nM when no stimuli were encountered, but changes in bacterial behavior correlated with changes in cytoplasmic free-Ca2+ concentration. For chemotactically wild-type E. coli, addition of a repellent resulted in cells tumbling and a transient increase in cytoplasmic free-Ca2+ levels. Conversely, addition of an attractant to wild-type cells caused running and produced a transient decrease in cytoplasmic free-Ca2+ levels. Studies with mutant strains showed that the chemoreceptors were required for the observed changes in cytoplasmic free-Ca2+ levels in response to chemical stimuli.

Publication Date

11-7-1995

Journal Title

Proceedings of the National Academy of Sciences

Publisher

National Academy of Sciences

Digital Object Identifier (DOI)

https://dx.doi.org/10.1073/pnas.92.23.10777

Document Type

Article

Comments

This is an article published by National Academy of Sciences in Proceedings of the National Academy of Sciences in 1995, available online: https://dx.doi.org/10.1073/pnas.92.23.10777

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