Cooperative expression of monocyte chemoattractant protein 1 within the bovine corpus luteum: Evidence of immune cell-endothelial cell interactions in a coculture system

Authors

Amy R. Liptak, University of New Hampshire
Brian T. Sullivan, University of New HampshireFollow
Luiz E. Henkes, Massachusetts General Hospital/Harvard Medical School
Missaka P.B. Wijayagunawardane, University of Peradeniya
Akio Miyamoto, Obihiro University of Agriculture and Veterinary Medicine
John S. Davis, University Nebraska Medical Center and Veterans Affairs Medical CenterFollow
Bo R. Rueda, Massachusetts General Hospital/Harvard Medical SchoolFollow
David H. Townson, University of New HampshireFollow

Abstract

Endothelial cells (EC) of the bovine corpus luteum (CL) are a known source of proinflammatory mediators, including monocyte chemoattractant protein 1 (CCL2) and endothelin 1 (EDN1). Here, a coculture system was devised to determine if immune cells and PGF(2 alpha) together affect CCL2 and EDN1 secretion by EC. Luteal EC were cultured either alone or together with peripheral blood mononuclear cells (PBMC), and treated without or with PGF(2 alpha) for 48 h (n = 6 experiments). Coculture of EC with PBMC increased CCL2 secretion an average of 5-fold higher compared with either cell type alone (P 0.05). EC cocultured with concanavalin A-activated PBMC (ActPBMC) increased CCL2 secretion an average of 12-fold higher compared with controls (P 0.05). Interestingly, PGF(2 alpha) did not alter either CCL2 or EDN1 secretion, regardless of culture conditions (P > 0.05). In a second series of experiments (n = 3 experiments), mixed luteal cells (MLC) were cultured alone or with PBMC as described above. Secretion of CCL2 and EDN1 was not affected by co-culture or by PGF(2 alpha) (P > 0.05), but MLC produced less progesterone in the presence of ActPBMC (P < 0.05). Collectively, these results suggest that immune cells and EC can interact cooperatively to increase CCL2 secretion in the CL, but this interaction does not affect EDN1 secretion nor is it influenced by PGF(2 alpha). Additionally, activated immune cells appear to produce a factor that impairs progesterone production by luteal steroidogenic cells.

Department

Molecular, Cellular and Biomedical Sciences

Publication Date

5-1-2005

Journal Title

Biology of reproduction

Publisher

Society for the Study of Reproduction

Digital Object Identifier (DOI)

10.1095/biolreprod.104.032953

Document Type

Article

Recommended Citation

Liptak A.R., ,Sullivan, B.T., Henkes, L.E., Wijayagunawardane, M.P.B., Miyamoto, A., Davis, J.S., Rueda, B.R., Townson, D.H. Cooperative expression of monocyte chemoatractant protein 1 within the bovine corpus luteum: Evidence of immune cell-endothelial cell interactions in a coculture system. Biol Reprod 72: 1169-1176 (2005).

Rights

© 2005 by the Society for the Study of Reproduction, Inc.