https://dx.doi.org/10.1104/pp.110.163394">
 

Title

Homology modeling of nitrogenase iron proteins from three Frankia strains

Abstract

The NifH protein contains an iron-sulfur cluster performing different functions during nitrogen fixation. Frankia is an actinomycete, entering into symbiotic association with a number of dicotyledonous plants and fixing nitrogen. The structure of the Frankia NifH protein was determined using homology modelling technique. Metal binding sites and functionally important regions of the protein were analyzed. Thiol ligands and active sites help in protein functioning and conformations. Structurally important nests were recognized. Clefts and cavities contain biologically important residues. Site-directed mutagenesis results reveal that mutations in functional residues hamper nitrogen fixation. The structure is rigid with an accessible surface for solvents. The structure is reliable offering insights into the 3D structural framework as well as structure-function relation of NifH protein.

Publication Date

1-1-2010

Journal Title

Symbiosis

Publisher

Springer

Digital Object Identifier (DOI)

https://dx.doi.org/10.1104/pp.110.163394

Document Type

Article

Rights

© Springer Science+Business Media B.V. 2009

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