We demonstrate a method for developing populations suitable for genome-wide high-resolution genetic linkage mapping, by recurrent intermating among F2 individuals derived from crosses between homozygous parents. Comparison of intermated progenies to F2 and “recombinant inbred” (RI) populations from the same pedigree corroborate theoretical expectations that progenies intermated for four generations harbor about threefold more information for estimating recombination fraction between closely linked markers than either RI-selfed or F2 individuals (which are, in fact, equivalent in this regard) . Although intermated populations are heterozygous, homozygous “intermated recombinant inbred” (IN) populations can readily be generated, combining additional information afforded by intermating with the permanence of RI populations. Intermated populations permit fine-mapping of genetic markers throughout a genome, helping to bridge the gap between genetic map resolution and the DNA-carrying capacity of modern cloning vectors, thus facilitating merger of genetic and physical maps. Intermating can also facilitate high-resolution mapping of genes and QTLs, accelerating map based cloning. Finally, intermated populations will facilitate investigation of other fundamental genetic questions requiring a genome-wide high-resolution analysis, such as comparative mapping of distantly related species, and the genetic basis of heterosis.
Sin-Chieh Liu, Stanley P. Kowalski, Tien-Hung Lan, Kenneth A. Feldmann & Andrew H. Paterson, "Genome-wide high-resolution mapping by recurrent intermating using Arabidopsis thaliana as a model" 142 Genetics 247 (1996), available at http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1206953/pdf/ge1421247.pdf