The efficiency of a Millitube MF cartridge filter, a membrane filter, for recovery of poliovirus from 100-gal volumes of both fresh (tap) and estuarine water was determined. In the high multiplicity of virus input-output experiments, recovery of 97% or greater of input virus was achieved in both types of water when the final concentration of divalent cation as Mg2+ was 1,200 μg/ml and the pH was 4.5. Virus was effectively eluted from the membrane cartridge with 5× nutrient broth in 0.05 M carbonate-bicarbonate buffer at pH 9.0. Four elutions of 250 ml each were used. In the low multiplicity of virus input-output experiments under the same cationic and pH conditions, up to 67% of the input virus was recovered when the virus was further concentrated from the eluates by the aqueous polymer two-phase separation technique. The volume reduction was 126,000-190,000 to 1. The use of the combined techniques, i.e., membrane adsorption followed by aqueous polymer two-phase separation, provided a highly sensitive, simple, and remarkably reliable sequential methodology for the quantitative recovery of poliovirus occurring at multiplicities as low as 1 to 2 plaque-forming units per 5 gal of water.
American Society for Microbiology
Hill, W.F., Jr., E.W. Akin, W.H. Benton and T.G. Metcalf. 1972. Virus in Water. II. Evaluation of membrane cartridge filters for recovering low multiplicities of poliovirus from water. Applied Microbiology 23:880-888.
Copyright © 1972 American Society for Microbiology