
Honors Theses and Capstones
Date of Award
Fall 2024
Project Type
Senior Honors Thesis
College or School
COLSA
Department
Biological Sciences
Program or Major
Zoology
Degree Name
Bachelor of Science
First Advisor
Jessica Bolker
Second Advisor
Janet Anderson
Abstract
This protocol outlines a enhanced clearing and staining procedure for persevered specimens, focusing on the visualization of cartilage and bone in small taxa. With origins from O. Shultze's diaphonization methodology in 1897, the process of clearing and staining improves the clarity and contrast of intricate tissues. It is essential for studies in developmental biology, histology and anatomy. This protocol covers the key steps of the process while detailing the materials, solutions and estimated timeline for each step pertaining to specific taxa. The aim of the protocol is to standardize and provide guidance for procedures in the Biological Sciences department at the University of New Hampshire.
Recommended Citation
Smith, Sadie E., "An Updated Outline for Clearing and Staining of Preserved Specimens" (2024). Honors Theses and Capstones. 868.
https://scholars.unh.edu/honors/868
Included in
Developmental Biology Commons, Integrative Biology Commons, Systems and Integrative Physiology Commons, Zoology Commons
Comments
Specimens used in the creation of this procedure are of the following species: Domestic chicken (Gallus Domesticus), Axolotl (Ambystoma mexicanum), House Mouse (Mus musculus), Brown Anole (Anolis sagrei), and non-venomous snake embryos. Specimens were taken from UNH collections previously reviewed by IACUC and approved for research.