Electrochemical and Spectroscopic Study of Peptide-template Melanins
Date of Award
Senior Honors Thesis
College or School
Program or Major
Bachelor of Science
Young Jo Kim
Melanins are organic polymers made up of 5,6-dihydroxyindole carboxylic acid (DHICA) and 5,6-dihydroxyindole (DHI). Biochemical systems synthesize melanins through a complex reaction pathway. Previous research exhibited that naturally-occurring melanins (NatMel) and their synthetic analogues (SynMel) can potentially serve as electrode materials for aqueous charge storage devices. Small surface area creates technical challenges for these melanins as charge storage materials. This research herein demonstrates that the structure of melanins can affect the charge storage capacities of monovalent Na+cations and divalent Mg2+ cations. 2-D sheet-like peptide melanins (PepMel) were synthesized into tripeptides consisting of aspartic acid (D), phenylalanine (F), and tyrosine (Y) through a heating and cooling process. Tyrosinase from Agaricus bisporus was used to oxidize the Y residues and initiate polymerization of the tripeptides into molecules with 2-D planar morphologies. Structural analysis of NatMel, SynMel, and PepMel was performed with scanning electron microscopy (SEM) and Brunauer-Emmett-Teller (BET) surface area measurement. Melanins were electrochemically examined by cyclic voltammetry and chronoamperometry followed by chemical analysis using Raman spectroscopy.
Courter, Daniel; Rossi, Olivia; and Tian, Zhen, "Electrochemical and Spectroscopic Study of Peptide-template Melanins" (2018). Honors Theses and Capstones. 398.