Analytical Ultracentrifugation: A Powerful ‘New’ Technology
Analytical ultracentrifugation (AUC) is a powerful means of characterizing the solution behavior of molecules. Sedimentation velocity analysis, the preferred AUC technique for characterizing complex systems, has higher resolution, broader applicable range and fewer solute/solvent limitations than gel-permeation chromatography. The technique is simple to perform and should become a mainstay for target identification, target validation, lead optimization, formulation in drug development and QA/QC. Recent studies have used AUC to characterize the binding stoichiometry and binding sites of an anti-tumor agent; of a hemoglobin-stabilizing protein, and of a fibril growth inhibitor, and to assess the causes of protein aggregation. The recent addition of fluorescence to the existing absorbance and interference detectors dramatically extends the flexibility of analytical ultracentrifugation.
Molecular, Cellular and Biomedical Sciences
Drug Discovery Today: Technologies
Digital Object Identifier (DOI)
Laue, T.M. (2004) "Analytical Ultracentrifugation: A Powerful ‘New’ Technology" in Drug Discovery Today: Technologies, 1:309-315.