Date of Award

Winter 1991

Project Type


Program or Major


Degree Name

Doctor of Philosophy

First Advisor

Sterling A Tomellini


An interactive, computer-assisted approach for determining multi-segment gradient elution profiles for High Performance Liquid Chromatography (HPLC) is presented. The approach is based on determining the gradient segment necessary to elute each solute at a user specified retention time. There are five main functions of this approach: (1) To inform the user of the possible retention time ranges for the solute that is going to be eluted for each elution step under both isocratic and gradient elution conditions; (2) To determine the required elution conditions, based on the user's desired retention times and the gradient elution shape; (3) To provide the user with a simulated chromatogram; (4) To calculate the required elution profile to provide for the 'zone compression'; and (5) To determine simplified multi-segment elution profiles.

The experimental and predicted separations using this approach were found to be in good agreement.