This DATSETNAMEreadme.txt file was generated on 2022-12-05 by Castine Bernardy GENERAL INFORMATION 1. Title of Dataset: Data supporting "Impact of Surface Properties on Viral Inactivation by UV254 Disinfection 2. Author Information A. Principal Investigator Contact Information Name: Castine Bernardy Institution: University of New Hampshire Address: 35 Colovos Road, Durham, NH 03824 Email: Castine.Bernardy@unh.edu B. Associate or Co-investigator Contact Information Name: James Malley Institution: University of New Hampshire Address: 35 Colovos Road, Durham, NH 03824 Email: Jim.Malley@unh.edu C. Alternate Contact Information Name: Institution: Address: Email: 3. Date of data collection (single date, range, approximate date): 2022-06-01---2022-10-15 4. Geographic location of data collection: Univeristy of New Hampshire Campus- Gregg Hall. 35 Colovos Road, Durham, NH 03824 5. Information about funding sources that supported the collection of the data: UNH Grant Codes: 14B411 and 1ddJM2 SHARING/ACCESS INFORMATION 1. Licenses/restrictions placed on the data: None. 2. Links to publications that cite or use the data: None. 3. Links to other publicly accessible locations of the data: None. 4. Links/relationships to ancillary data sets: None. 5. Was data derived from another source? No A. If yes, list source(s): 6. Recommended citation for this dataset: Bernardy, C., Malley, J. Data Supporting Impact of Surface Properties on Viral Inactivation by UV254 Disinfection [dataset]. UNH Scholars Repository. DATA & FILE OVERVIEW 1. File List: This data set contains all of the data used to support the claims made in the article Impact of Surface Properties on Viral Inactivation by UV254 Disinfection. The data set contains multiople tabs including; aluminum, ceramic, Formica laminate, PTFE, stainless steel, controls, contact angle, surface roughness, porosity, ddPCR, irradiance map. 2. Relationship between files, if important: 3. Additional related data collected that was not included in the current data package: 4. Are there multiple versions of the dataset? No. A. If yes, name of file(s) that was updated: i. Why was the file updated? ii. When was the file updated? METHODOLOGICAL INFORMATION Please see the methods sections of the Evaluating the Efficacy of UV254 Disinfection by Surface Type for futher details. 1. Description of methods used for collection/generation of data: UV254 experiments were conducted on five surface types: aluminum, ceramic, Formica laminate, PTFE and stainless steel. The surrogate used for all experiments was MS-2 bacteriophage. The virus was innoculated onto the surfaces with a cotton swab. After the desired UV dose, the surfaces were rinsed with 50 mL of sterile PBS, the wash water was collected and set out for analysis. 2. Methods for processing the data: Plaque assay was the primary microbial technique used for MS-2 enumeration. For select samples, ddPCR was utlized. Prior to running ddPCR, the Powermicrobiome RNeasy kit was used for RNA extraction. The data was reported as PFU/mL or copies/mL. 3. Instrument- or software-specific information needed to interpret the data: The following were used to determine the characteristics of the surfaces. Contact Angle: Biolin Scientfiic ThetaLite 101 tensiometer. The OneAttension software was then used to record these data. Surface Roughness: Olympus LEXT OLS5000 SD Laser Confocalo Microscope. Porosity: A scanning electron microscpe (SEM) was used capture images of the surfaces at 100,000 times magnification. The NIS-Elements 5.14.01 software was used to quantify the deperessions (pore spaces) in each SEM image. Irradiance map: An IL1700 radiometer was used to collect the irradiance measurements, which were entered into Microscoft excel to create the irradiance map. RNA extraction: QIAGEN QIAcube. ddPCR: Thermocycler, Droplet Reader, Droplet Generator (QIAGEN). 4. Standards and calibration information, if appropriate: To ensure the accuracy of each UV dose, an irradiance map was created to determine the spread of irradiance on the surfaces. A weighted average was calcualted and was used to determine the exposure time required for each dose. 5. Environmental/experimental conditions: The experiments were conducted in a controlled temperature room in a Gregg Hall laboratory. The temperature was set to 28 degrees Celsius and relative humidity at 55% (Dew point: 18 degrees Celsius). The conditions were closely monitored with a humidity and temperature probe. 6. Describe any quality-assurance procedures performed on the data: Many steps were taken for QA/QC purposes, and are listed below. Concentration checks: Each experimental day, a sample of the stock MS-2 solution was sent out for analysis. This was to determine the quality of the viral titer and ensure that the cocnetration values were holding steady. Negative controls: These controls were conducted with aluminum, cermaic and PTFE. Sterile PBS was inoculated onto the surfaces with a cotton swab and rinsed off using 50 mL of PBS (as per the procedure). This ensured that residual MS-2 from previous experiments were not a source of contamination. Positive controls: These controls were conducted for surfaces (in triplicate). For this experiment, the surface was inoculated with MS-2 (with the cotton swab) and rinsed per the procedure, without exposure to UV (0mJ/cm2). This experiment determined the effects on the virus that were strictly due to the unqiue surface characteristics. Irradiance check: Each experimental day, the irradiance was checked on the Trojan UV colliamted beam to ensure that the lamp was functioning properly. 3 trials: The dose response curve for each surface was tested in triplicates to identify potential outliers in the data. Time exposure tests: When unsure about the causal mechanisms of high inactivation at low UV doses (PTFE), a study was conducted to ensure that the virus was not effected by dessicationon the surface. Autoclave between experiments: After each experiment, all glassware was autoclaved to minimize risk of contaimination. In addition, the metal surfaces were autoclaved, whereas the remainder of them were treated with a chlorine bath and rinsed thoroughly afterwards. 7. People involved with sample collection, processing, analysis and/or submission: Castine Bernardy, Jim Malley, Jia Choi, Nora Sinno. DATA-SPECIFIC INFORMATION FOR: [FILENAME] 1. Number of variables: 2. Number of cases/rows: 3. Variable List: All experiments and corresponding data are clearly spelled out in the data file. 4. Missing data codes: n 5. Specialized formats or other abbreviations used: