https://dx.doi.org/10.3835/plantgenome2015.08.0071">
 

Abstract

Fragaria iinumae Makino is recognized as an ancestor of the octoploid strawberry species, which includes the cultivated strawberry, Fragaria ×ananassa Duchesne ex Rozier. Here we report the construction of the first high-density linkage map for F. iinumae. The F. iinumae linkage map (Fii map) is based on two high-throughput techniques of single nucleotide polymorphism (SNP) genotyping: the IStraw90 Array (hereafter “Array”), and genotyping by sequencing (GBS). The F2 generation mapping population was derived by selfing F. iinumae hybrid F1D, the product of a cross between two divergent F. iinumae accessions collected from Hokkaido, Japan. The Fii map consists of seven linkage groups (LGs) and has an overall length of 451.7 cM as defined by 496 loci populated by 4173 markers: 3280 from the Array and 893 from GBS. Comparisons with two versions of the Fragaria vesca ssp. vesca L. ‘Hawaii 4’ pseudo-chromosome (PC) assemblies reveal substantial conservation of synteny and colinearity, yet identified differences that point to possible genomic divergences between F. iinumae and F. vesca, and/or to F. vesca genomic assembly errors. The Fii map provides a basis for anchoring a F. iinumae genome assembly as a prerequisite for constructing a second diploid reference genome for Fragaria.

Publication Date

3-28-2016

Journal Title

The Plant Genome

Publisher

ACSESS (Alliance of Crop, Soil, and Environmental Science Societies)

Digital Object Identifier (DOI)

https://dx.doi.org/10.3835/plantgenome2015.08.0071

Scientific Contribution Number

2619

Document Type

Article

Rights

Copyright © 2016 Crop Science Society of America

Comments

This is an article published by ACSESS (Alliance of Crop, Soil, and Environmental Science Societies) in The Plant Genome in 2016, available online: https://dx.doi.org/10.3835/plantgenome2015.08.0071

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