Title

Using nuclear and organelle DNA markers to discriminate among Picea rubens, Picea mariana, and their hybrids

Abstract

The pattern of nuclear ribosomal alleles in controlled-cross black spruce × red spruce hybrids was compared with that of samples from provenance tests of the entire range of red spruce (Picearubens Sarg.) and the eastern complex of black spruce (Piceamariana (Mill.) BSP). In addition, restriction fragment length polymorphisms were identified for the organelle genomes of red spruce and black spruce. Organelle haplotypes were closely associated with species, but were not species specific. Data from controlled-cross hybrids suggest that mitochondria are maternally inherited and chloroplasts are paternally inherited in these spruce species. Organelle markers were combined with restriction fragment length polymorphism data from the nuclear rDNA repeat to derive a simple three-character index capable of identifying red spruce, black spruce, and hybrids of the two species.

Publication Date

3-1996

Journal Title

Canadian Journal of Forest Research

Publisher

Canadian Science Publishing

Digital Object Identifier (DOI)

10.1139/x26-049

Scientific Contribution Number

1750

Document Type

Article